BioNano genome mapping service at Kansas State University
Background and fees:
We are a fee-for service center with a Irys automated imaging system from BioNano Genomics. We generate physical maps of your genomes. To learn more about how the maps are generated visit the BioNano Genomics website.
We can start with tissue samples, or high molecular weight DNA in plugs of low melt agarose. We are working on a fee schedule that separates each step.
|Description of the products or services||Amount Internal||Amount External|
|Data analysis and training||50.00/hr||75.00/hr|
|Computer information specialist/data management, mining,software support||76.00/hr||114.00/hr|
|Outreach coordinator/data analysis, experimental design||70.00/hr||104.00/hr|
|Genomic map/ sample prep||each||220||330|
|Genomic map/ HMW stabilization||each||190||283|
|Genomic map/Quantification and QC||each||165||247|
|Genomic map/NickLabelRepair rxn||each||245||368|
|Genomic map/Imaging/per flowcell||each||533||800|
For a ~100Mb genome we would start with 1 flow cell, larger genomes require more flow cells. Some clients purchase a BioRad DNA plug kit (cat no. 170-3593) and perform step 1 themselves (then send us HMW DNA in plugs), we will provide detailed protocols and advice with BioNano input.) If the genome is large, we would have to perform additional NLR rxns and run more flow cells.
Request an estimate:
In order to prepare an estimate for generating your genome map on the Irys automated imaging system from BioNano Genomics we will need the following information about your organism. Send emails to firstname.lastname@example.org:
- Scientific name of your organism (required)
- Name of project PI (required)
- Email address (required)
- Sequence data (required)
- Fasta file of your genome assembly and (if available) your scaffolds
- If sequence data is not available we can estimate nicking rate based on a close relative. NCBI has a genome database. Please send the link to a closely related genome with the most complete genome.
- If available, descriptive statistics of your genome and/or assembly (e.g. text files with number of contigs/scaffolds and cumulative length of assembly, or flow cytometry estimates of genome size).